标题: Minimally Invasive 3D Laser Printing of Microimplants Inside Living Organisms [打印本页] 作者: admin 时间: 2026-11-4 20:08 标题: Minimally Invasive 3D Laser Printing of Microimplants Inside Living Organisms Source: Additive Manufacturing Technology (3D Printing) Branch, Chinese Mechanical Engineering Society.
Contributors: Liu Zhe and Gao Lin, National Key Laboratory of Precision Micro/Nano Manufacturing Technology, Xi'an Jiaotong University.
Multiphoton 3D laser printing has attracted interest as a way to make biocompatible scaffolds that modify and guide cell behavior in vitro. In-vivo tissue engineering has so far been limited to implanting preprinted biocompatible scaffolds or bioprinting tissue structures using bioinks containing cells, biomolecules, and printable hydrogel formulations. A comprehensive platform for in-vivo, in-situ laser printing of synthetic-polymer microimplants has therefore been lacking.
Cassian Afting's team used 3D laser printing to fabricate artificial microimplants inside developing organisms' organs. They selected medaka fish and Drosophila melanogaster, which have short generation times, long histories in genetic research, and transparent eggs suitable for observation and experimental handling. After microinjecting ink into fruit-fly embryo yolk or developing optic vesicles of medaka embryos, the embryos received a one-minute UV pulse for global exposure and photocrosslinking. An external light source from a conventional fluorescence microscope cured the injected ink during photopolymerization. The team successfully printed stars and hollow cylinders inside organisms.
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Figure 1. Workflow for biocompatible single-photon photopolymerization and multiphoton 3D laser printing in early living organisms: schematic of 1PP and 2PP workflows in early Drosophila and medaka embryos.
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Figure 2. a) Mounting method for embryo laser printing. b) Microscopy images of star-shaped 3D laser printing after IP-PDMS microinjection into early fruit-fly embryos. Printed structures were manually dissected from developing embryo yolk and imaged using a stereomicroscope. c) Mounting method for medaka embryo printing. d) IP-PDMS microinjection into a fish embryo followed by multiphoton printing of a hollow cylinder.
The team outlined a single-photon platform and successfully performed 3D laser printing in fruit-fly and medaka embryos. It demonstrated microinjection, UV curing, and single- and multiphoton polymerization and printing of polydimethylsiloxane in extracellular tissue environments. This provides a feasible approach to minimally invasive placement of biocompatible, nondegradable microimplants in vivo.
Reference:
Afting C, Mainik P, Martel V C, et al. Minimal-Invasive 3D Laser Printing of Microimplants in Organismo. Advanced Science (Weinheim, Baden-Wurttemberg, Germany), 2024, e2401110.
AI-translated from an article by Xiaoruanxiong on Nanjixiong, originally published on 2026-11-03. Text and images retain the original source attribution. Read the original Chinese article
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